Quantitative determination of urea concentrations in cell culture medium.
نویسندگان
چکیده
Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplate-based, room temperature assay can be applied to determine urea synthesis in cell culture.
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عنوان ژورنال:
- Biochemistry and cell biology = Biochimie et biologie cellulaire
دوره 87 3 شماره
صفحات -
تاریخ انتشار 2009